Isolation and Characterization of Extracellular Vesicles Produced by Iron-limited Mycobacteria
Title:
Isolation and Characterization of Extracellular Vesicles Produced by Iron-limited Mycobacteria
Author:
Gupta, Shamba
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (507 seconds)
Series:
Immunology and Infection
General Note:
Title from resource description page
Abstract:
Mycobacterium tuberculosis shows increased production and release of extracellular vesicles in response to low iron conditions. This work details a protocol for generating low iron conditions and methods for the purification and characterization of mycobacterial extracellular vesicles released in response to iron deficiency.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/60359