Silencing the Spark: CRISPR/Cas9 Genome Editing in Weakly Electric Fish
Title:
Silencing the Spark: CRISPR/Cas9 Genome Editing in Weakly Electric Fish
Author:
Constantinou, Savvas J.
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (480 seconds)
Series:
Biology
General Note:
Title from resource description page
Abstract:
Here, a protocol is presented to produce and rear CRISPR/Cas9 genome knockout electric fish. Outlined in detail are the required molecular biology, breeding, and husbandry requirements for both a gymnotiform and a mormyrid, and injection techniques to produce Cas9-induced indel F0 larvae.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/60253