Identifying Mutations by High Resolution Melting in a TILLING Population of Rice
Title:
Identifying Mutations by High Resolution Melting in a TILLING Population of Rice
Author:
Li, Shan
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (370 seconds)
Series:
Genetics
General Note:
Title from resource description page
Abstract:
In this article, we present the protocol that is described as high-resolution melting analysis (HRM)-based Target Induced Local Lesions In Genomes (TILLING). This method utilizes fluorescence changes during the melting of the DNA duplex and is suitable for high-throughput screening of both insertion/deletion (Indel) and single base substition (SBS).
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/59960