Serum Free Production of Three-dimensional Human Hepatospheres from Pluripotent Stem Cells
Title:
Serum Free Production of Three-dimensional Human Hepatospheres from Pluripotent Stem Cells
Author:
Lucendo-Villarin, Balta
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (417 seconds)
Series:
Developmental Biology
General Note:
Title from resource description page
Abstract:
This protocol describes an approach to produce hepatospheres from human pluripotent stem cells using a defined culture system and cell self-assembly. This protocol is reproducible in a number of cell lines, cost effective and allows the production of stable human hepatospheres for biomedical application.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/59965