Mitochondria and Endoplasmic Reticulum Imaging by Correlative Light and Volume Electron Microscopy
Title:
Mitochondria and Endoplasmic Reticulum Imaging by Correlative Light and Volume Electron Microscopy
Author:
Jung, Minkyo
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (561 seconds)
Series:
Biochemistry
General Note:
Title from resource description page
Abstract:
We present a protocol to study the distribution of mitochondria and endoplasmic reticulum in whole cells after genetic modification using correlative light and volume electron microscopy including ascorbate peroxidase 2 and horseradish peroxidase staining, serial sectioning of cells with and without the target gene in the same section, and serial imaging via electron microscopy.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/59750