Pan-lyssavirus Real Time RT-PCR for Rabies Diagnosis
Title:
Pan-lyssavirus Real Time RT-PCR for Rabies Diagnosis
Author:
Marston, Denise A.
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (385 seconds)
Series:
Immunology and Infection
General Note:
Title from resource description page
Abstract:
This real-time RT-PCR using dsDNA intercalating dye is suitable to diagnose lyssavirus infections. The method begins with RNA extracted from rabies suspected ante-mortem or post-mortem samples, detailing master mix preparation, RNA addition, setup of the real-time machine and correct interpretation of results.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/59709