Assessing Cell Viability and Death in 3D Spheroid Cultures of Cancer Cells
Title:
Assessing Cell Viability and Death in 3D Spheroid Cultures of Cancer Cells
Author:
Rolver, Michala G.
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (633 seconds)
Series:
Cancer Research
General Note:
Title from resource description page
Abstract:
Here, we present several simple methods for evaluating viability and death in 3D cancer cell spheroids, which mimic the physico-chemical gradients of in vivo tumors much better than the 2D culture. The spheroid model, therefore, allows evaluation of the cancer drug efficacy with improved translation to in vivo conditions.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/59714