Efficient Differentiation of Human Pluripotent Stem Cells into Liver Cells
Title:
Efficient Differentiation of Human Pluripotent Stem Cells into Liver Cells
Author:
Loh, Kyle M.
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (457 seconds)
Series:
Developmental Biology
General Note:
Title from resource description page
Abstract:
This protocol details a monolayer, serum-free method to efficiently generate hepatocyte-like cells from human pluripotent stem cells (hPSCs) in 18 days. This entails six steps as hPSCs sequentially differentiate into intermediate cell-types such as the primitive streak, definitive endoderm, posterior foregut and liver bud progenitors before forming hepatocyte-like cells.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/58975