Tissue Preparation and Immunostaining of Mouse Craniofacial Tissues and Undecalcified Bone
Title:
Tissue Preparation and Immunostaining of Mouse Craniofacial Tissues and Undecalcified Bone
Author:
Yang, Jingwen
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (603 seconds)
Series:
Developmental Biology
General Note:
Title from resource description page
Abstract:
Here, we present a detailed protocol to detect and quantify protein levels during craniofacial morphogenesis/pathogenesis by immunostaining using mouse craniofacial tissues as examples. In addition, we describe a method for preparation and cryosectioning of undecalcified hard tissues from young mice for immunostaining.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/59113