Fully Human Tumor-based Matrix in Three-dimensional Spheroid Invasion Assay
Title:
Fully Human Tumor-based Matrix in Three-dimensional Spheroid Invasion Assay
Author:
Naakka, Erika
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (495 seconds)
Series:
Cancer Research
General Note:
Title from resource description page
Abstract:
Tumor microenvironment is an essential part of cancer growth and invasion. To mimic carcinoma progression, a biologically relevant human matrix is needed. This protocol introduces an improvement for the in vitro three-dimensional spheroid invasion assay by applying a human leiomyoma-based matrix. The protocol also introduces a computer-based cell invasion analysis.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/59567