Bacterial Cell Culture at the Single-cell Level Inside Giant Vesicles
Title:
Bacterial Cell Culture at the Single-cell Level Inside Giant Vesicles
Author:
Morita, Masamune
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (453 seconds)
Series:
Immunology and Infection
General Note:
Title from resource description page
Abstract:
We demonstrate single-cell culture of bacteria inside giant vesicles (GVs). GVs containing bacterial cells were prepared by the droplet transfer method and were immobilized on a supported membrane on a glass substrate for direct observation of bacterial growth. This approach may also be adaptable to other cells.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/59555