Localization of SUMO-modified Proteins Using Fluorescent Sumo-trapping Proteins
Title:
Localization of SUMO-modified Proteins Using Fluorescent Sumo-trapping Proteins
Author:
Yin, Rui
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (383 seconds)
Series:
Biochemistry
General Note:
Title from resource description page
Abstract:
SUMO is an essential and highly conserved, small ubiquitin-like modifier protein. In this protocol we are describing the use of a stress-tolerant recombinant SUMO-trapping protein (kmUTAG) to visualize native, untagged SUMO conjugates and their localization in a variety of cell types.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/59576