Fluorescent Silver Staining of Proteins in Polyacrylamide Gels
Title:
Fluorescent Silver Staining of Proteins in Polyacrylamide Gels
Author:
Wong, Alex Y. H.
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (384 seconds)
Series:
Biochemistry
General Note:
Title from resource description page
Abstract:
Here, we describe a detailed protocol outlining a new fluorescent staining technique for total protein detection in polyacrylamide gels. The protocol utilizes a silver ion-specific fluorescence turn-on probe, which detects Ag+-protein complexes, and eliminates certain limitations of traditional chromogenic silver stains.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/58669