Generation of 3D Skin Organoid from Cord Blood-derived Induced Pluripotent Stem Cells
Title:
Generation of 3D Skin Organoid from Cord Blood-derived Induced Pluripotent Stem Cells
Author:
Kim, Yena
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (594 seconds)
Series:
Developmental Biology
General Note:
Title from resource description page
Abstract:
We propose a protocol that shows how to differentiate induced pluripotent stem cell-derived keratinocytes and fibroblasts and generate a 3D skin organoid, using these keratinocytes and fibroblasts. This protocol contains an additional step of generating a humanized mice model. The technique presented here will improve dermatologic research.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/59297