Generation of Monoclonal Antibodies Against Natural Products
Title:
Generation of Monoclonal Antibodies Against Natural Products
Author:
Zhang, Yue
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (735 seconds)
Series:
Immunology and Infection
General Note:
Title from resource description page
Abstract:
This article provides a detailed protocol for the preparation and evaluation of monoclonal antibodies against natural products for use in various immunoassays. This procedure includes immunization, cell fusion, indirect competitive ELISA for positive clone screening, and monoclonal hybridoma preparation. The specifications for antibody characterization using MALDI-TOF-MS and ELISA analyses are also provided.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/57116