Preparation and Gene Modification of Nonhuman Primate Hematopoietic Stem and Progenitor Cells
Title:
Preparation and Gene Modification of Nonhuman Primate Hematopoietic Stem and Progenitor Cells
Author:
Radtke, Stefan
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (676 seconds)
Series:
Genetics
General Note:
Title from resource description page
Abstract:
The goal of this protocol is to isolate nonhuman primate CD34+ cells from primed bone marrow, to gene-modify these cells with lentiviral vectors, and to prepare a product for infusion into the autologous host. The total protocol length is approximately 48 h.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/58933