RNA-based Reprogramming of Human Primary Fibroblasts into Induced Pluripotent Stem Cells
Title:
RNA-based Reprogramming of Human Primary Fibroblasts into Induced Pluripotent Stem Cells
Author:
McGrath, Patrick S.
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (698 seconds)
Series:
Developmental Biology
General Note:
Title from resource description page
Abstract:
Here we describe a clinically relevant, high-efficiency, feeder-free method to reprogram human primary fibroblasts into induced pluripotent stem cells using modified mRNAs encoding reprogramming factors and mature microRNA-367/302 mimics. Also included are methods to assess reprogramming efficiency, expand clonal iPSC colonies, and confirm expression of the pluripotency marker TRA-1-60.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/58687