Expression and Purification of Mammalian Bestrophin Ion Channels
Title:
Expression and Purification of Mammalian Bestrophin Ion Channels
Author:
Kittredge, Alec
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (492 seconds)
Series:
Biochemistry
General Note:
Title from resource description page
Abstract:
The purification of ion channels is often challenging, but once achieved, it can potentially allow in vitro investigations of the functions and structures of the channels. Here, we describe the stepwise procedures for the expression and purification of mammalian bestrophin proteins, a family of Ca2+-activated Cl- channels.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/57832