Visualization of DNA Compaction in Cyanobacteria by High-voltage Cryo-electron Tomography
Title:
Visualization of DNA Compaction in Cyanobacteria by High-voltage Cryo-electron Tomography
Author:
Murata, Kazuyoshi
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (587 seconds)
Series:
Biology
General Note:
Title from resource description page
Abstract:
This protocol describes how to visualize the transient DNA compaction in cyanobacteria. Synchronous cultivation, monitoring by fluorescence microscopy, rapid freezing, and high voltage cryo-electron tomography are used. A protocol for these methodologies is presented, and future applications and developments are discussed.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/57197