Counting Proteins in Single Cells with Addressable Droplet Microarrays
Title:
Counting Proteins in Single Cells with Addressable Droplet Microarrays
Author:
Chatzimichail, Stelios
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (745 seconds)
Series:
Cancer Research
General Note:
Title from resource description page
Abstract:
Here we present addressable droplet microarrays (ADMs), a droplet array based method able to determine absolute protein abundance in single cells. We demonstrate the capability of ADMs to characterize the heterogeneity in expression of the tumor suppressor protein p53 in a human cancer cell line.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/56110