Biotinylated Cell-penetrating Peptides to Study Intracellular Protein-protein Interactions
Title:
Biotinylated Cell-penetrating Peptides to Study Intracellular Protein-protein Interactions
Author:
Jaraíz-Rodríguez, Myriam
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (626 seconds)
Series:
Biochemistry
General Note:
Title from resource description page
Abstract:
This is a protocol to study intracellular protein-protein interactions based on the biotin-avidin pull-down system with the novelty of combining cell-penetrating sequences. The main advantage is that the target sequence is incubated with living cells instead of cell lysates and therefore the interactions will occur within the cellular context.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/56457