Sample Preparation for Endopeptidomic Analysis in Human Cerebrospinal Fluid
Title:
Sample Preparation for Endopeptidomic Analysis in Human Cerebrospinal Fluid
Author:
Hansson, Karl T.
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (623 seconds)
Series:
Neuroscience
General Note:
Title from resource description page
Abstract:
A method for mass spectrometric analysis of endogenous peptides in human cerebrospinal fluid (CSF) is presented. By employing molecular weight cut-off filtration, chromatographic pre-fractionation, mass spectrometric analysis and a subsequent combination of peptide identification strategies, it was possible to expand the known CSF peptidome nearly ten-fold compared to previous studies.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/56244