Differentiation of Mouse Embryonic Stem Cells into Cortical Interneuron Precursors
Title:
Differentiation of Mouse Embryonic Stem Cells into Cortical Interneuron Precursors
Author:
Tischfield, David J.
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (624 seconds)
Series:
Neuroscience
General Note:
Title from resource description page
Abstract:
This protocol describes a method for generating cortical interneuron progenitors and post-mitotic interneuron precursors from mouse embryonic stem cells using a modified embryoid body-to-monolayer method. These progenitors/precursors can be used in vitro or fluorescently sorted and transplanted into neonatal neocortex for studying fate determination, or used in therapeutic applications.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/56358