Sample Preparation for Mass Spectrometry-based Identification of RNA-binding Regions
Title:
Sample Preparation for Mass Spectrometry-based Identification of RNA-binding Regions
Author:
Warneford-Thomson, Robert
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (652 seconds)
Series:
Biochemistry
General Note:
Title from resource description page
Abstract:
We describe a protocol to identify RNA-binding proteins and map their RNA-binding regions in live cells using UV-mediated photocrosslinking and mass spectrometry.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/56004