In Vitro Polymerization of F-actin on Early Endosomes
Title:
In Vitro Polymerization of F-actin on Early Endosomes
Author:
Muriel, Olivia
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (735 seconds)
Series:
Biochemistry
General Note:
Title from resource description page
Abstract:
Early endosome functions depend on F-actin polymerization. Here, we describe a microscopy-based in vitro assay that reconstitutes the nucleation and polymerization of F-actin on early endosomal membranes in test tubes, thus rendering this complex series of reactions amenable to biochemical and genetic manipulations.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/55829