Integration Free Derivation of Human Induced Pluripotent Stem Cells Using Laminin 521 Matrix
Title:
Integration Free Derivation of Human Induced Pluripotent Stem Cells Using Laminin 521 Matrix
Author:
Uhlin, Elias
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (648 seconds)
Series:
Developmental Biology
General Note:
Title from resource description page
Abstract:
Robust derivation of human induced pluripotent stem (hiPS) cells was achieved by using non-integrating Sendai virus (SeV) vector mediated reprogramming of dermal fibroblasts. hiPS cell maintenance and clonal expansion was performed using xeno-free and chemically defined culture conditions with recombinant human laminin 521 (LN-521) matrix and Essential E8 (E8) Medium.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/56146