In Situ Immunofluorescent Staining of Autophagy in Muscle Stem Cells
Title:
In Situ Immunofluorescent Staining of Autophagy in Muscle Stem Cells
Author:
Castagnetti, Francesco
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (515 seconds)
Series:
Biology
General Note:
Title from resource description page
Abstract:
Active autophagy is associated with productive muscle regeneration, which is essential for Muscle Stem Cell (MuSC) activation. Here, we provide a protocol for the in situ detection of LC3, an autophagy marker in MyoD-positive MuSCs of muscle tissue sections from control and injured mice.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/55908