Voltage-clamp Fluorometry in Xenopus Oocytes Using Fluorescent Unnatural Amino Acids
Title:
Voltage-clamp Fluorometry in Xenopus Oocytes Using Fluorescent Unnatural Amino Acids
Author:
Kalstrup, Tanja
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (564 seconds)
Series:
Biochemistry
General Note:
Title from resource description page
Abstract:
This article describes an enhancement of conventional Voltage-Clamp Fluorometry (VCF) where Fluorescent Unnatural Amino Acids (fUAA) are used instead of maleimide dyes, to probe structural rearrangements in ion channels. The procedure includes Xenopus oocyte DNA injection, RNA/fUAA coinjection, and simultaneous current and fluorescence measurements.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/55598