Measuring Protein Binding to F-actin by Co-sedimentation
Title:
Measuring Protein Binding to F-actin by Co-sedimentation
Author:
Heier, Jonathon A.
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (377 seconds)
Series:
Biochemistry
General Note:
Title from resource description page
Abstract:
This protocol describes a method to test the ability of a protein to co-sediment with filamentous actin (F-actin) and, if binding is observed, to measure the affinity of the interaction.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/55613