High Precision FRET at Single-molecule Level for Biomolecule Structure Determination
Title:
High Precision FRET at Single-molecule Level for Biomolecule Structure Determination
Author:
Ma, Junyan
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (684 seconds)
Series:
Biochemistry
General Note:
Title from resource description page
Abstract:
A protocol for high-precision FRET experiments at the single molecule level is presented here. Additionally, this methodology can be used to identify three conformational states in the ligand-binding domain of the N-methyl-D-aspartate (NMDA) receptor. Determining precise distances is the first step towards building structural models based on FRET experiments.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/55623