Flash-and-Freeze: A Novel Technique to Capture Membrane Dynamics with Electron Microscopy
Title:
Flash-and-Freeze: A Novel Technique to Capture Membrane Dynamics with Electron Microscopy
Author:
Li, Shuo
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (601 seconds)
Series:
Biology
General Note:
Title from resource description page
Abstract:
We developed a novel technique in electron microscopy, "flash-and-freeze," that enables the visualization of membrane dynamics with ms temporal resolution. This technique combines the optogenetic stimulation of neurons with high-pressure freezing. Here, we demonstrate the procedures and describe the protocols in detail.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/55664