A G-quadruplex DNA-affinity Approach for Purification of Enzymatically Active G4 Resolvase1
Title:
A G-quadruplex DNA-affinity Approach for Purification of Enzymatically Active G4 Resolvase1
Author:
Routh, Eric D.
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (685 seconds)
Series:
Biochemistry
General Note:
Title from resource description page
Abstract:
G4 Resolvase1 binds to G-quadruplex (G4) structures with the tightest reported affinity for a G4-binding protein and represents the majority of the G4-DNA unwinding activity in HeLa cells. We describe a novel protocol that harnesses the affinity and ATP-dependent unwinding activity of G4-Resolvase1 to specifically purify catalytically active recombinant G4R1.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/55496