In Vitro Differentiation of Human Pluripotent Stem Cells into Trophoblastic Cells
Title:
In Vitro Differentiation of Human Pluripotent Stem Cells into Trophoblastic Cells
Author:
Wang, Jianle
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (501 seconds)
Series:
Developmental Biology
General Note:
Title from resource description page
Abstract:
Here, we present a protocol to efficiently generate human trophoblastic cells from human pluripotent stem cells using bone morphogenic protein 4 and inhibitors of the Activin/Nodal pathways. This method is suitable for the efficient differentiation of human pluripotent stem cells and can generate large quantities of cells for genetic manipulation.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/55268