Generation of Murine Monoclonal Antibodies by Hybridoma Technology
Title:
Generation of Murine Monoclonal Antibodies by Hybridoma Technology
Author:
Holzlöhner, Pamela
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (582 seconds)
Series:
Immunology and Infection
General Note:
Title from resource description page
Abstract:
An optimized protocol is presented for the generation of monoclonal antibodies based on the hybridoma technology. Mice were immunized with an immunoconjugate. Spleen cells were fused by PEG and an electric impulse with immortal myeloma cells. Antibody-producing hybridoma cells were selected by HAT and antigen-specific ELISA screening.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/54832