Immunostaining of Biocytin-filled and Processed Sections for Neurochemical Markers
Title:
Immunostaining of Biocytin-filled and Processed Sections for Neurochemical Markers
Author:
Swietek, Bogumila
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (534 seconds)
Series:
Neuroscience
General Note:
Title from resource description page
Abstract:
This protocol presents a method for the morphological recovery of neurons patched during electrophysiological recordings using biocytin filling and subsequent immunohistochemical postprocessing. We show that thick biocytin-filled sections that were stained and coverslipped can be restained with a second primary antibody days or months later.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/54880