Generation of Human Monocyte-derived Dendritic Cells from Whole Blood
Title:
Generation of Human Monocyte-derived Dendritic Cells from Whole Blood
Author:
Posch, Wilfried
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (455 seconds)
Series:
Immunology and Infection
General Note:
Title from resource description page
Abstract:
Here, we demonstrate how monocytes are isolated by magnetic bead separation from peripheral blood mononuclear cells after density gradient centrifugation of human anti-coagulated blood. Following incubation for 5 days, human monocytes are differentiated into immature dendritic cells and are ready for experimental procedures in a non-clinical setting.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/54968