Efficient and Site-specific Antibody Labeling by Strain-promoted Azide-alkyne Cycloaddition
Title:
Efficient and Site-specific Antibody Labeling by Strain-promoted Azide-alkyne Cycloaddition
Author:
Kim, Sanggil
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (546 seconds)
Series:
Biochemistry
General Note:
Title from resource description page
Abstract:
Here, we present a protocol to site-specifically introduce chemical probes into an antibody fragment by genetically incorporating an azide-containing amino acid, and subsequently coupling the azide with a chemical probe by strain-promoted azide-alkyne cycloaddition (SPAAC).
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/54922