Induced Pluripotent Stem Cell Generation from Blood Cells Using Sendai Virus and Centrifugation
Title:
Induced Pluripotent Stem Cell Generation from Blood Cells Using Sendai Virus and Centrifugation
Author:
Rim, Yeri Alice
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (597 seconds)
Series:
Developmental Biology
General Note:
Title from resource description page
Abstract:
We propose a protocol for reprogramming peripheral blood mononuclear cells (PBMCs) into induced pluripotent stem cells (iPSCs). By plating the transduced blood cells onto matrix-coated plates with centrifugation, iPSCs are successfully induced from floating cells. This technique suggests a simple and effective reprogramming protocol for cells such as PBMCs and CBMCs.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/54650