Protein Complex Affinity Capture from Cryomilled Mammalian Cells
Title:
Protein Complex Affinity Capture from Cryomilled Mammalian Cells
Author:
LaCava, John
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (637 seconds)
Series:
Biochemistry
General Note:
Title from resource description page
Abstract:
Here we describe protocols to disrupt mammalian cells by solid-state milling at a cryogenic temperature, produce a cell extract from the resulting cell powder, and isolate protein complexes of interest by affinity capture upon antibody-coupled micron-scale paramagnetic beads.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/54518