Multi-enzyme Screening Using a High-throughput Genetic Enzyme Screening System
Title:
Multi-enzyme Screening Using a High-throughput Genetic Enzyme Screening System
Author:
Kim, Haseong
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (490 seconds)
Series:
Biology
General Note:
Title from resource description page
Abstract:
This work presents a method of high-throughput screening using a universal genetic enzyme screening system that can be theoretically applied to over 200 enzymes. Here, the single screening system identifies three different enzymes (lipase, cellulase, and alkaline phosphatase) by simply changing the substrate used (p-nitrophenyl acetate, p-nitrophenyl-β-D-cellobioside, and phenyl phosphate).
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/54059