Preparation and Applications of Organotypic Thymic Slice Cultures
Title:
Preparation and Applications of Organotypic Thymic Slice Cultures
Author:
Sood, Aditi
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (610 seconds)
Series:
Immunology and Infection
General Note:
Title from resource description page
Abstract:
We describe the preparation of thymic slices that, in combination with flow cytometry, can be used to model positive and negative selection of developing T cells. Thymic slices can also be adapted for the in situ analysis of thymocyte migration, localization, and signaling via immunofluorescence and two-photon microscopy.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/54355