Generation of Integration-free Human Induced Pluripotent Stem Cells Using Hair-derived Keratinocytes
Title:
Generation of Integration-free Human Induced Pluripotent Stem Cells Using Hair-derived Keratinocytes
Author:
Hung, Sandy S.C.
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (516 seconds)
Series:
Developmental Biology
General Note:
Title from resource description page
Abstract:
This manuscript provides a step-by-step procedure for the derivation and maintenance of human keratinocytes from plucked hair and subsequent generation of integration-free human induced pluripotent stem cells (hiPSCs) by episomal vectors.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/53174