Alginate Encapsulation of Pluripotent Stem Cells Using a Co-axial Nozzle
Title:
Alginate Encapsulation of Pluripotent Stem Cells Using a Co-axial Nozzle
Author:
Horiguchi, Ikki
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (433 seconds)
Series:
Developmental Biology
General Note:
Title from resource description page
Abstract:
We established a method of encapsulating pluripotent stem cells (PS cells) into alginate hydrogel capsules using a co-axial nozzle. This prevents cells from aggregating excessively and limits the shear stress experienced by cells in suspension culture. The technique is applicable to the mass production of PS cells as well as research on stem cell niche.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/52835