Pairwise Growth Competition Assay for Determining the Replication Fitness of Human Immunodeficiency Viruses
Title:
Pairwise Growth Competition Assay for Determining the Replication Fitness of Human Immunodeficiency Viruses
Author:
Manocheewa, Siriphan
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (679 seconds)
Series:
Immunology and Infection
General Note:
Title from resource description page
Abstract:
Growth competition between nearly isogenic viruses provides a sensitive measurement for determining relative replication fitness. The protocols described here include the construction of recombinant HIV-1 clones, virus propagation and growth competition and analysis methods optimized to yield sensitive and consistent results.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/52610