An Enzymatic Method to Rescue Mesenchymal Stem Cells from Clotted Bone Marrow Samples
Title:
An Enzymatic Method to Rescue Mesenchymal Stem Cells from Clotted Bone Marrow Samples
Author:
Schlaefli, Philipp
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (538 seconds)
Series:
Developmental Biology
General Note:
Title from resource description page
Abstract:
Mesenchymal stem cells are usually obtained from bone marrow and require expansion culture. When samples clot before processing, a protocol using the (enzymatic) thrombolytic drug urokinase can be applied to degrade the clot. Thus, cells are released and available for expansion culture. This protocol provides a rapid and inexpensive alternative to resampling.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/52694