Isolation and Functional Analysis of Mitochondria from Cultured Cells and Mouse Tissue
Title:
Isolation and Functional Analysis of Mitochondria from Cultured Cells and Mouse Tissue
Author:
Lampl, Thomas
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (567 seconds)
Series:
Biology
General Note:
Title from resource description page
Abstract:
Comparison of mitochondrial membrane potential between samples yields valuable information about cellular status. Detailed steps for isolating mitochondria and assessing response to inhibitors and uncouplers using fluorescence are described. The method and utility of this protocol are illustrated by use of a cell culture and animal model of cellular stress.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/52076