Mammosphere Formation Assay from Human Breast Cancer Tissues and Cell Lines
Title:
Mammosphere Formation Assay from Human Breast Cancer Tissues and Cell Lines
Author:
Lombardo, Ylenia
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (651 seconds)
Series:
Medicine
General Note:
Title from resource description page
Abstract:
Floating mammosphere assays can investigate the subset of stem-like breast cancer cells that survive in suspension conditions and show enhanced tumorigenesis when implanted into mice. This protocol provides a convenient in vitro measure of sphere-forming ability, a proxy for in vivo tumorigenesis, while facilitating analysis of the stem-associated transcriptional landscape.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/52671