Isolation and Enrichment of Human Adipose-derived Stromal Cells for Enhanced Osteogenesis
Title:
Isolation and Enrichment of Human Adipose-derived Stromal Cells for Enhanced Osteogenesis
Author:
Zielins, Elizabeth R.
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (660 seconds)
Series:
Developmental Biology
General Note:
Title from resource description page
Abstract:
The transcriptional heterogeneity within human adipose-derived stromal cells can be defined on the single cell level using cell surface markers and osteogenic genes. We describe a protocol utilizing flow cytometry for the isolation of cell subpopulations with increased osteogenic potential, which may be used to enhance craniofacial skeletal reconstruction.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/52181