A Guide to Modern Quantitative Fluorescent Western Blotting with Troubleshooting Strategies
Title:
A Guide to Modern Quantitative Fluorescent Western Blotting with Troubleshooting Strategies
Author:
Eaton, Samantha L.
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (661 seconds)
Series:
Biology
General Note:
Title from resource description page
Abstract:
The advancement of western blotting using fluorescence has allowed detection of subtle changes in protein expression enabling quantitative analyses. Here we describe a robust methodology for detection of a range of proteins across a variety of species and tissue types. A strategy to overcome common technical problems is also provided.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/52099