Production and Targeting of Monovalent Quantum Dots
Title:
Production and Targeting of Monovalent Quantum Dots
Author:
Seo, Daeha
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (616 seconds)
Series:
Bioengineering
General Note:
Title from resource description page
Abstract:
We provide detailed instructions for the preparation of monovalent targeted quantum dots (mQDs) from phosphorothioate DNA of defined length. DNA wrapping occurs in high yield, and therefore, products do not require purification. We demonstrate the use of the SNAP tag to target mQDs to cell-surface receptors for live-cell imaging applications.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/52198